Green bugs some moving
Our cell biological work on parasite organelles and their function and development depends heavily on light microscopic imaging approaches. We use specific antibodies as well as tagged transgenes to localize molecules within the parasite cell. The use of fluorescent proteins allows us to follow cellular processes in real time or time lapse by video microscopy. You will need apple quicktime to watch the mov movie files on this site. We are happy for you to use our images for teaching and research but please note that we hold the copyright and ask you to contact us if you wish to publish our images.
| Toxoplasma cell division: : | ||
Toxoplasma divides by internal budding, preforming two daughters within each mother cell. Phase contras time lapse over several hours showing replication of intracellular parasites. |
Molecular components of the parasite budding machinary (IMC red, MORN1 green, DNA blue)
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| Molecular components of the parasite budding machinary (IMC green, nucleus (histone) red) ras, Chennai, India | Two T. gondii parasites during mitosis and budding. The nuclei are labeled with Histone-RFP and the centrocones/spindle and the developing daughter rings in green (MORN1-YFP) | |
| The apicoplast a residual parasite chloroplast : | nair@uga.edu | |
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| Each Toxoplasma parasite contains a single apicoplast (here shown by ACP-GFP labeling, note that plastids have begun the devision process). | Each Toxoplasma parasite contains a single apicoplst (plastids labeled using FNR-RFP, cytoplasm labeled with YFP-YFP) |
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striepen@cb.uga.edu
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| In Sarcocystis neurona the apicoplast is a single continuous organelle throughout the development of the schizont and fission and segregation concurs with the formation of multiple daughters (plastid, green, DNA, blue, micronemes, red). | As in Toxoplasma in Sarcocystis the apicoplast shows tight association with the centrosomes (apicoplast, green, tubulin (spindles), red, DNA, blue. |
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| Fluorescent protein labeling of various parasite compartments: | giel@uga.edu
706-583-0589 |
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| P30-GFP is secreted into the parasitophorous vacuole via dense granules. | Worked in the lab as a grad student on apicoplast metabolism and graduated with a PhD. Now postdoc with Celeste Simon University of Pennsylvania and HHMI, Philadelphia. jollymazumdar@gmail.com |
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| Shipra Vaishnava, M.S. from Maharaja Sayajirao University, Baroda, India | Worked in the lab as a graduate student on the replication and division of the apicoplast and graduated with a PhD. Now postdoc with Laura Hooper at UT Southwestern in Dallas. |
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| Marc-Jan Gubbels, Ph.D. from Utrecht University, Utrecht, The Netherlands | Worked in the lab as a postdoc on Toxoplasma genetics and cell biology. Now Assistant Professor in the Department of Biology, Boston College, Boston, MA 617-552-8722 |
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| Nwakaso Umejiego, B.S. from Univeristy of Georgia, | Worked in the lab on Cryptosporidium IMPDH as an undergrad researcher, graduated with a B.S. and stayed on for a year as a lab tech. Now Medical Student at Harvard School of Medicine, Boston, MA |
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| David Morrison, M.S. from UGA | Worked in the lab on apicomplexan cell division, graduated with a M.S. Works as a Peace Corps Vounteer in Jamaica in an AIDS prevention program. |
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| Chitra Kanchagar, M.S. from Univeristy of Georgia | Worked in the lab on the Trypanosome IMPDH and graduated with a Masters. Labcoordinator of the Lipshutz lab, University of Pennsylvania School of Medicine, Philadelphia, PA |
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| Catherine Li, B.S. from University of Washington | Worked in the lab as labcoordinator on Toxoplasma complementation and Cryptosporidium nucleotide metabolism. Labcoordinator of the Morrissette lab, Department of Molecular Biology & Biochemistry, UC Irvine , Irvine, CA catli98@yahoo.com |
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| Nico Boot | Worked in the lab on Toxoplasma genetic tools and graduated with a Masters from the University of Amsterdam. PhD student with Dr. Van den Ackeren, Dep. of Biology, Univeristy of Utrecht, Utrecht, The Netherlands |
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| Andrea Pruijssers | Worked in the lab on Cryptosporidium nucleotide metabolism and graduated with a Masters from the University of Wageningen. PhD student with Dr. Strand, Dep. Entomology, UGA andrea_pruijssers@hotmail.com |
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| Marnix Wieffer | Worked in the lab on Toxoplasma genetic tools and graduated with a Masters from the University of Utrecht. PhD student with Dr. Van de Sluijs, Institute for Biomembranes, Univeristy of Utrecht, Utrecht, The Netherlands |
